←
Browse All Journals Submit Manuscript
Skip to content
Home▶Archive▶Vol. 38 No. 2 (2021)▶DNA EXTRACTION FROM FORMALIN FIXED TISSUE USING AN…
Journal of Forensic Medicine and Toxicology ›
Print ISSN 0971-1929 · Online ISSN 0974-4568
Submit your paper About the journal
Original Research Paper

DNA EXTRACTION FROM FORMALIN FIXED TISSUE USING AN ALTERNATIVE PROTOCOL

, , ,
K Chauhan — Senior Scientific Officer, Biology division, Central Forensic Science Laboratory (CBI), CGO Complex, Lodhi Road, New Delhi, India
BK Mohapatra — Senior Scientific Assistant, Biology division, Central Forensic Science Laboratory (CBI), New Delhi, India
R Kaushik — PhD Student, DNA Laboratory, AIIMS, New Delhi
C Behera — Laboratory technician, DNA Laboratory, AIIMS, New Delhi
Published 7 Jun 2024 Vol. 38 No. 2 (2021), pp. 78-80
❝ How to cite
Formats:PDF 🔒 Subscription
🔒 PDF

Abstract

It is always a challenging task for obtaining desired amount of amplifiable DNA from formalin-fixed and non paraffin-embedded tissue sample for the purpose of human individualization, since formalin fixation of tissue samples causes fragmentation of the DNA and formation of cross linked protein-DNA complexes. The present study sought laboratories in cases related to product of conception,abandoned babies, microtomized and stained histo- pathological slides etc. needing DNA test.

KeywordsFormalin fixed tissueDNA Extractionde crosslinkingPCR amplificationDNA profiling
🔒 Full text available to subscribers
  • PDF — print-ready version
Log in Subscribe Institutional access
  1. Gilbert MT, Haselkorn T, Bunce M, Sanchez JJ, Lucas SB, Jewell LD, Van Marck E, Worobey M. The isolation of nucleic acids from fixed, paraffin embedded tissues—which methods are useful when? PLoS ONE.2007;2: e537.
  2. Gillio-Tos A, De Marco L, Fiano V, Garcia-Bragado F, Dikshit R, Boffetta P, Merletti F. Efficient DNA extraction from 25-year-old paraffin-embedded tissues: study of 365 samples. Pathology. 2007; 39:345–348.
  3. Pavelic J, Gall-Troselj K, Bosnar MH, Kardum MM, Pavelic K. PCR amplification of DNA from archival specimens. A methodological approach. Neoplasma. 1996; 43:75–81.
  4. Santos MC, Saito CP, Line SR. Extraction of genomic DNA from paraffin-embedded tissue sections of human fetuses fixed and stored in formalin for long periods. Pathol Res Pract. 2008; 204:633–636.
  5. Shi SR, Datar R, Liu C, Wu L, Zhang Z, Cote RJ, Taylor CR. DNA extraction from archival formalin fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution. Histochem Cell Biol 2004; 122:211–218.
  6. Wright DK, Manos MM. Sample preparation from paraffin-embedded tissues. In: Innis MA, Gelfand DH, Sninsky JJ, et al, eds. PCR protocols: a guide to methods and applications. London: Academic Press, 1990:153–8.
  7. CaoW, Hashibe M, Rao JY, Morgenstern H, Zhang ZF. Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells. Cancer Detect Prev. 2003; 27:397–404.
  8. Coombs NJ, Gough AC, Primrose JN. Optimization of DNA and RNA extraction from archival formalin fixed tissue. Nucleic Acids Res. 1999; 27: e12.

Looking up citing articles…